Human Calcium-sensing R/CaSR Antibody

Catalog #: MAB9608 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9608-100
MAB9608-SP

Detection of Calcium-sensing R/CaSR in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Calcium-sensing R/CaSR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Calcium-sensing R/CaSR protein in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 611825

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived transfected with recombinant human Calcium-sensing R/CaSR protein
Tyr20-Lys601
Accession # P41180-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Calcium-sensing R/CaSR antibody in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP antibody by Flow Cytometry.

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Detection of Calcium-sensing R/CaSR in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Calcium-sensing R/CaSR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Calcium-sensing R/CaSR Monoclonal Antibody (Catalog # MAB9608) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcium-sensing R/CaSR

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

References

Geng, Y. et al. (2016) Elife 5:e13662.
Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
Brown, E. M. et al. (1993) Nature 366:575.
Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.

Long Name

Calcium-sensing Receptor

Entrez Gene IDs

846 (Human); 12374 (Mouse); 24247 (Rat)

Alternate Names

calcium-sensing receptor; CAR; CaSR; EIG8; extracellular calcium-sensing receptor; FHH; FIH; GPRC2A; GPRC2AHHC; HHC1; hypocalciuric hypercalcemia 1; NSHPT; parathyroid Ca(2+)-sensing receptor 1; Parathyroid cell calcium-sensing receptor; PCaR1; PCAR1MGC138441