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Human Caspase-10 Antibody

Catalog #: MAB834
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB834
MAB834-SP
Detection of Human Caspase‑10 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspase-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspase-10 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 63131
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human Caspase-10/a/B (Mch 4)
Val220-Ile479
Accession # AAC50644
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from ascites

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Caspase-10 antibody by Western Blot. View Larger

Detection of Human Caspase‑10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-10 Monoclonal Antibody (Catalog # MAB834), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-10 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-10

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43  generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Entrez Gene IDs
843 (Human)
Alternate Names
Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase10; Caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2

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Citations for Human Caspase-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Identification of caspase-10 in human neutrophils and its role in spontaneous apoptosis.
    Authors: Goepel F, Weinmann P, Schymeinsky J, Walzog B
    J. Leukoc. Biol., 2004-02-03;75(5):836-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Upregulation of the Fas receptor death-inducing signaling complex after traumatic brain injury in mice and humans.
    Authors: Qiu J, Whalen MJ, Lowenstein P, Fiskum G, Fahy B, Darwish R, Aarabi B, Yuan J, Moskowitz MA
    J. Neurosci., 2002-05-01;22(9):3504-11.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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