Human CCL1/I‑309/TCA‑3 Biotinylated Antibody Summary
Lys24-Lys96
Accession # P22362
Applications
Human CCL1/I-309 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3
Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.
CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15-16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.
- Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
- Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Product Datasheets
Citations for Human CCL1/I‑309/TCA‑3 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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CC chemokine ligand 1 is released into the airways of atopic asthmatics.
Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
Eur. Respir. J., 2006-03-15;28(1):59-67.
Species: Human
Sample Types: BALF
Applications: ELISA Development -
A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
J. Leukoc. Biol., 2001-10-01;70(4):617-23.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.
