Human CCL2/JE/MCP-1 Antibody

Name: Human CCL2/JE/MCP-1 Antibody
Brand: R&D Systems
SKU: MAB2791
Rating: 5 (1 reviews)

Catalog #: MAB2791
9 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2791-SP
MAB2791-100
MAB2791-500

CCL2/JE/MCP‑1 in Human Breast Cancer Tissue.

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All CCL2/JE/MCP-1 Antibodies

Product Details

Citations (9)

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Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL2/JE/MCP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.

Source

Monoclonal Mouse IgG_2B Clone # 23002

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CCL2/MCP-1 Gln24-Thr99 Accession # P13500

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CCL2/JE/MCP‑1 in Human Breast Cancer Tissue. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB2791) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

MCP-1 is a beta -chemokine that has been designated CCL2. It is produced by a variety of cell types and is chemotactic for monocytes.

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Alternate Names

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL2/JE/MCP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape
Authors: LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman
J Am Heart Assoc, 2020-11-16;9(23):e017094.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense
Authors: M Chen, RR Reed, AP Lane
Cell Stem Cell, 2019-09-12;25(4):501-513.e5.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Estrogen promotes progression of hormone-dependent breast cancer through CCL2-CCR2 axis by upregulation of Twist via PI3K/AKT/NF-?B signaling
Authors: R Han, S Gu, Y Zhang, A Luo, X Jing, L Zhao, X Zhao, L Zhang
Sci Rep, 2018-06-22;8(1):9575.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Authors: Rump L, Mattey D, Kehoe O, Middleton J
Cytokine, 2017-09-01;97(0):133-140.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
Nature, 2014-10-22;515(7525):130-3.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
Am. J. Transplant., 2008-07-01;8(7):1512-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
Authors: Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A
Endocrinology, 2008-01-10;149(4):1678-86.
Species: Human
Sample Types: Whole Cells
Applications: ICC
The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
Authors: Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
Am. J. Pathol., 2007-11-30;171(6):1789-99.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load.
Authors: Pulliam L, Sun B, Rempel H
J. Neuroimmunol., 2004-12-01;157(1):93-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC