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Human CCL2/JE/MCP-1 Antibody

Catalog #: MAB279
15 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB279-SP
MAB279-100
MAB279-500
Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL2/JE/MCP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5, 6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.
Source
Monoclonal Mouse IgG1 Clone # 24822
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL2/JE/MCP-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human CCL2/JE/MCP-1 antibody by Western Blot. View Larger

Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody. View Larger

Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody. Recombinant Human CCL2/JE/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND50 is typically 0.9-4.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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Citations for Human CCL2/JE/MCP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Melatonin Modulates the Microenvironment of Glioblastoma Multiforme by Targeting Sirtuin 1
    Authors: SW Lai, YS Liu, DY Lu, CF Tsai
    Nutrients, 2019-06-14;11(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Continuous Delivery of Neutralizing Antibodies Elevate CCL2 Levels in Mice Bearing MCF10CA1d Breast Tumor Xenografts
    Authors: M Yao, C Smart, Q Hu, N Cheng
    Transl Oncol, 2017-07-19;10(5):734-743.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. CoREST1 promotes tumor formation and tumor stroma interactions in a mouse model of breast cancer.
    Authors: Mazumdar, Sohini, Arendt, Lisa M, Phillips, Sarah, Sedic, Maja, Kuperwasser, Charlott, Gill, Grace
    PLoS ONE, 2015-03-20;10(3):e0121281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
    Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
    J Neurosci, 2015-02-11;35(6):2452-64.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012-09-17;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
    Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
    Int. J. Cancer, 2010-03-01;126(5):1095-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009-02-08;30(4):313-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008-02-01;214(3):328-36.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ELISA Development, IHC-Fr
  9. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007-10-01;13(19):5719-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  10. Induction of host chemotactic response by Encephalitozoon spp.
    Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
    Infect. Immun., 2006-12-18;75(4):1619-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2006-10-04;192(1):56-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Systemic inflammation in nonalcoholic fatty liver disease is characterized by elevated levels of CCL2.
    Authors: Haukeland JW, Damas JK, Konopski Z, Loberg EM, Haaland T, Goverud I, Torjesen PA, Birkeland K, Bjoro K, Aukrust P
    J. Hepatol., 2006-03-20;44(6):1167-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
    Authors: Penninckx F, Ectors N
    Exp. Cell Res., 2005-02-15;303(2):331-42.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. The CC chemokine ligand, CCL2/MCP1, participates in macrophage fusion and foreign body giant cell formation.
    Authors: Kyriakides TR, Foster MJ, Keeney GE, Tsai A, Giachelli CM, Clark-Lewis I, Rollins BJ, Bornstein P
    Am. J. Pathol., 2004-12-01;165(6):2157-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  15. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004-01-23;75(3):504-14.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry

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Reviews for Human CCL2/JE/MCP-1 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human CCL2/JE/MCP-1 Antibody
By Anonymous on 11/07/2021
Application: WB Sample Tested: Glioblastoma Species: Human
Western Blot Human CCL2/JE/MCP-1 Antibody MAB279
Human CCL2/JE/MCP-1 Antibody
By Zoia Levashova on 11/20/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC).
Paired with SulfoTag-modified AF-279-NA as a detection antibody. Calibration curves with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)

Other Human CCL2/JE/MCP-1 Antibody MAB279
Human CCL2/JE/MCP-1 Antibody
By Balaji Mahender on 12/20/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human
Human CCL2/JE/MCP-1 Antibody
By Anonymous on 11/20/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used with BAF279 to create a sandwich ELISA to measure MCP-1 in human serum samples.

ELISA Human CCL2/JE/MCP-1 Antibody MAB279