Human CCL3/CCL4 Fluorescein-conjugated Antibody Summary
Ala27-Ala92
Accession # P10147
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CCL3/CCL4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL3/CCL4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2701F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL3/CCL4
The Macrophage Inflammatory Proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines.
Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
Product Datasheets
Citations for Human CCL3/CCL4 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Trained immunity in newborn infants of HBV-infected mothers.
Authors: Hong M, Sandalova E, Low D, Gehring A, Fieni S, Amadei B, Urbani S, Chong Y, Guccione E, Bertoletti A
Nat Commun, 2015-03-25;6(0):6588.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, IHC-Fr -
Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
Authors: Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR
Vaccine, 2010-02-23;28(8):1932-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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