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Human CCL4/MIP-1 beta Fluorescein-conjugated Antibody

Catalog #: IC271F
12 Citations Datasheet / COA / SDS
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IC271F
Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry.
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Citations (12)
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Human CCL4/MIP-1 beta Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
Source
Monoclonal Mouse IgG2B Clone # 24006
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CCL4/MIP‑1 beta antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP-1 beta Fluorescein-conjugated Monoclonal Antibody (Catalog # IC271F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CCL4/MIP-1 beta

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Entrez Gene IDs
6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)
Alternate Names
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

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Citations for Human CCL4/MIP-1 beta Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Preservation of Lymphopoietic Potential and Virus Suppressive Capacity by CD8+ T Cells in HIV-2-Infected Controllers
    J Immunol, 2016-08-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
    Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
    PLoS Pathog, 2015-03-24;11(3):e1004671.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD8+ regulatory T cells, and not CD4+ T cells, dominate suppressive phenotype and function after in vitro live Mycobacterium bovis-BCG activation of human cells.
    Authors: Boer M, van Meijgaarden K, Joosten S, Ottenhoff T
    PLoS ONE, 2014-04-08;9(4):e94192.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CD39 is involved in mediating suppression by Mycobacterium bovis BCG-activated human CD8(+) CD39(+) regulatory T cells.
    Authors: Boer M, van Meijgaarden K, Bastid J, Ottenhoff T, Joosten S
    Eur J Immunol, 2013-05-29;43(7):1925-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. A molecular basis for the control of preimmune escape variants by HIV-specific CD8+ T cells.
    Authors: Ladell K, Hashimoto M, Iglesias M, Wilmann P, McLaren J, Gras S, Chikata T, Kuse N, Fastenackels S, Gostick E, Bridgeman J, Venturi V, Arkoub Z, Agut H, van Bockel D, Almeida J, Douek D, Meyer L, Venet A, Takiguchi M, Rossjohn J, Price D, Appay V
    Immunity, 2013-03-21;38(3):425-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
    Authors: Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R
    PLoS Pathog, 2012-11-29;8(11):e1003042.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection.
    Authors: Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, O'Neil SP, Kaur A
    J. Immunol., 2011-03-25;186(9):5151-61.
    Species: Primate - Cercocebus torquatus (Sooty Mangabey)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes.
    Authors: Guzzo C, Che Mat NF, Gee K
    J. Biol. Chem., 2010-06-02;285(32):24404-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CD16- natural killer cells: enrichment in mucosal and secondary lymphoid tissues and altered function during chronic SIV infection.
    Authors: Reeves RK, Gillis J, Wong FE
    Blood, 2010-03-25;115(22):4439-46.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
    Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
    Blood, 2008-01-18;111(7):3579-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Chemokines and their receptors in whiplash injury: elevated RANTES and CCR-5.
    Authors: Kivioja J, Rinaldi L, Ozenci V, Kouwenhoven M, Kostulas N, Lindgren U, Link H
    J. Clin. Immunol., 2001-07-01;21(4):272-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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