Human CCL8/MCP-2 Antibody

Catalog #: AB-281-NA Datasheet / COA / SDS

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AB-281-NA

Chemotaxis Induced by CCL8/MCP‑2 and Neutral-ization by Human CCL8/ MCP‑2 Antibody.

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All CCL8/MCP-2 Antibodies

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Human CCL8/MCP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) MCP-1 and rhMCP-3 is observed. Neutralizes the biological activity of recombinant human MCP-2, but will not neutralize the biological activity of MCP-1 or MCP-3.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human CCL8/MCP-2
Gln24-Pro99
Accession # P80075

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Purity

Protein A or G purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)

Neutralization

Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in human monocytes. The Neutralization Dose (ND₅₀) is typically 60-120 µg/mL in the presence of 0.4 µg/mL Recombinant Human CCL8/MCP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL8/MCP‑2 and Neutral-ization by Human CCL8/ MCP‑2 Antibody. Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP-2 Polyclonal Antibody (Catalog # AB-281-NA). The ND₅₀ is typically 60-120 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL8/MCP-2

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Entrez Gene IDs

6355 (Human); 20307 (Mouse)

Alternate Names

C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8