Human CCR10 Antibody

Name: Human CCR10 Antibody
Brand: R&D Systems
SKU: MAB3478
Rating: 4 (1 reviews)

Catalog #: MAB3478
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3478
MAB3478-SP

Detection of CCR10 in Human Blood Lymphocytes by Flow Cytometry.

1 Image

All CCR10 Antibodies

Product Details

Citations (5)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR10 Antibody Summary

Species Reactivity

Human

Specificity

Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2A Clone # 314305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with human CCR10
Gln8-Asn362
Accession # P46092

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CCR10 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Rat Anti-Human CCR10 Monoclonal Antibody (Catalog # MAB3478) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Alternate Names

CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCR10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells.
Authors: McCully M, Collins P, Hughes T, Thomas C, Billen J, O'Donnell V, Moser B
J Immunol, 2015-05-22;195(1):96-104.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells.
Authors: Davey M, Morgan M, Liuzzi A, Tyler C, Khan M, Szakmany T, Hall J, Moser B, Eberl M
J Immunol, 2014-08-27;193(7):3704-3716.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
Authors: Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
PLoS ONE, 2014-08-27;9(8):e106008.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.
Authors: Duhen T, Campbell D
J Immunol, 2014-06-02;193(1):120-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression.
Authors: Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS
J. Allergy Clin. Immunol., 2007-10-01;121(1):148-157.e3.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry