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Human CCR4 Antibody

Catalog #: MAB1567
19 Citations Datasheet / COA / SDS
Clone 205410 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1567-SP
MAB1567-100
MAB1567-500
Detection of CCR4 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR4 Antibody Summary

Species Reactivity
Human
Specificity
Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects CCR4 on human PBLs and platelets.
Source
Monoclonal Mouse IgG2B Clone # 205410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CCR4 transfectants
Met1-Leu360
Accession # P51679
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Wong, M.T. et al. (2015) Cell Rep. 11: 1822. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR4 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR4

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Entrez Gene IDs
1233 (Human); 12773 (Mouse); 171054 (Rat); 403541 (Canine); 102120178 (Cynomolgus Monkey)
Alternate Names
C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5

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Citations for Human CCR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Identification of human CD4+ T cell populations with distinct antitumor activity
    Authors: MH Nelson, HM Knochelman, SR Bailey, LW Huff, JS Bowers, K Majchrzak-, MM Wyatt, MP Rubinstein, S Mehrotra, MI Nishimura, KE Armeson, PG Giresi, MJ Zilliox, HE Broxmeyer, CM Paulos
    Science Advances, 2020-07-01;6(27):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions
    Authors: M Martin-Fer, M Bravo Garc, C Gruber, S Murias Loz, MNH Malik, F Alsohime, A Alakeel, R Valdez, S Buta, G Buda, MA Marti, M Larralde, B Boisson, M Feito Rodr, X Qiu, M Chrabieh, M Al Ayed, S Al Muhsen, JV Desai, EMN Ferre, SD Rosenzweig, B Amador-Bor, LY Bravo-Gall, R Olmer, S Merkert, M Bret, AK Sood, A Al-Rabiaah, MH Temsah, R Halwani, M Hernandez, F Pessler, JL Casanova, J Bustamante, MS Lionakis, D Bogunovic
    Cell Rep, 2020-05-12;31(6):107633.
    Species: Human
    Sample Types:
    Applications: CyTof
  3. Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection
    Authors: D Michlmayr, EY Kim, AH Rahman, R Raghunatha, S Kim-Schulz, Y Che, S Kalayci, ZH Gümü?, G Kuan, A Balmaseda, A Kasarskis, SM Wolinsky, M Suaréz-Far, E Harris
    Cell Rep, 2020-04-28;31(4):107569.
    Species: Human
    Sample Types: Plasma
    Applications: CyTof
  4. Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model
    Authors: NM Bath, X Ding, NA Wilson, BM Verhoven, BA Boldt, A Sukhwal, SR Reese, SE Panzer, A Djamali, RR Redfield
    PLoS ONE, 2019-02-08;14(2):e0211865.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Optimization of mass cytometry sample cryopreservation after staining
    Authors: Evan W Newell
    Cytometry A, 2016-10-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  6. CD20+ B cell depletion alters T cell homing.
    Authors: Kap, Yolanda, van Driel, Nikki, Laman, Jon D, Tak, Paul P, 't Hart, Bert A
    J Immunol, 2014-04-02;192(9):4242-53.
    Species: Marmoset
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer.
    Authors: Aruga A, Takeshita N, Kotera Y, Okuyama R, Matsushita N, Ohta T, Takeda K, Yamamoto M
    Clin Cancer Res, 2013-03-11;19(8):2224-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2012-11-07;6(3):626-38.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  9. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011-01-09;12(2):167-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009-07-10;21(8):991-1001.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model.
    Authors: Nakamura M, Jo J, Tabata Y, Ishikawa O
    Am. J. Pathol., 2008-02-02;172(3):650-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007-09-15;179(6):3724-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007-03-29;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2006-12-04;319(1):1-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
    Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
    J. Immunol., 2006-05-15;176(10):5735-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
    Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
    Int. J. Oncol., 2004-09-01;25(3):605-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. A CCR2-V64I polymorphism affects stability of CCR2A isoform.
    Authors: Nakayama EE, Tanaka Y, Nagai Y, Iwamoto A, Shioda T
    AIDS, 2004-03-26;18(5):729-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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