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Human CCR6 APC-conjugated Antibody

Catalog #: FAB195A
4 Citations Datasheet / COA / SDS
Clone 53103 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB195A-025
FAB195A-100
Detection of CCR6 in Human PBMCs by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR6 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
Source
Monoclonal Mouse IgG2B Clone # 53103
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR6
Met1-Met374
Accession # P51684
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Purity
Protein A or G purified from ascites

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR6 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB195A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

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Citations for Human CCR6 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
    Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
    Arthritis Res. Ther., 2018-06-07;20(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes
    Authors: T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart
    Mol Metab, 2017-09-27;6(11):1529-1539.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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