Human CD117/c-kit Antibody

Catalog #: AF3267
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3267
AF3267-SP

Detection of Human CD117/c-kit by Western Blot.

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All CD117/c-kit Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD117/c-kit Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD117/c-kit in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Human CD117/c-kit synthetic peptide
Asp716-Pro726
Accession # P10721

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3267) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD117/c-kit at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human CD117/c-kit by Simple Western^TM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 172 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3267). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.

References

Broudy, V. (1997) Blood 90:1345.
Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citation for Human CD117/c-kit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Engineering Stem Cell Factor Ligands with Different c-Kit Agonistic Potencies
Authors: T Tilayov, T Hingaly, Y Greenshpan, S Cohen, B Akabayov, R Gazit, N Papo
Molecules, 2020-10-21;25(20):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot