Human CD14 PE-conjugated Antibody Summary
Thr20-Cys352
Accession # P08571
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of CD14 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD14
CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
- Wright, S.D. et al. (1990) Science 249:1431.
- Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
- Beutler, B. (2000) Current Opinion in Immunology 12:20.
- Stelter, F. (2000) Chem. Immunol. 74:25.
Product Datasheets
Citations for Human CD14 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia
Authors: B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha
Cancers (Basel), 2020-07-31;12(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
Authors: S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio
Biomed Res Int, 2020-07-27;2020(0):8748934.
Species: Human hepegivirus
Sample Types: Whole Cells
Applications: Flow Cytometry -
Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic CellsStabilization of Interferon Regulatory Factor 8
Authors: Z Agod, K Pazmandi, D Bencze, G Vereb, T Biro, A Szabo, E Rajnavolgy, A Bacsi, P Engel, A Lanyi
Front Immunol, 2018-01-26;9(0):62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming
Authors: H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar
J Transl Med, 2017-01-13;15(1):10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TLR4/CD14 Variants-Related Serologic and Immunologic Dys-Regulations Predict Severe Sepsis in Febrile De-Compensated Cirrhotic Patients
PLoS ONE, 2016-11-18;11(11):e0166458.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR4 positive and angiogenic monocytes in myocardial infarction.
Thromb Haemost, 2012-12-06;109(2):255-62.
Species: Human
Sample Types: Whole Blood
Applications: Flow Cytometry -
Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
PLoS ONE, 2012-10-09;7(10):e47187.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
Authors: Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
Cytometry A, 2011-07-06;79(8):635-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Epitope specificity and clonality of EBV-specific CTLs used to treat posttransplant lymphoproliferative disease.
Authors: McAulay KA, Haque T, Urquhart G, Bellamy C, Guiretti D, Crawford DH
J. Immunol., 2009-03-15;182(6):3892-901.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
J. Mol. Biol., 2006-12-28;367(1):25-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human CD14 PE-conjugated Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human CD14 PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
