Human CD155/PVR APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD155/PVR in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD155/PVR APC-conjugated Monoclonal Antibody (Catalog # FAB25301A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD155/PVR in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CD155/PVR APC-conjugated Monoclonal Antibody (Catalog # FAB25301A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 degreesC as supplied.
Background: CD155/PVR
CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60-65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6-8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.
- Takai, Y. et al. (2003) Cancer Sci. 94:655.
- Mendelsohn, C.L. et al. (1989) Cell 56:855.
- Koike, H. et al. (1990) EMBO J. 9:3217.
- Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
- Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
- Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
- Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
- Lange, R. et al. (2001) Virology 285:218.
Product Datasheets
Citation for Human CD155/PVR APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Tinostamustine (EDO-S101), an Alkylating Deacetylase Inhibitor, Enhances the Efficacy of Daratumumab in Multiple Myeloma by Upregulation of CD38 and NKG2D Ligands
Authors: Díaz-Tejedor, A;Rodríguez-Ubreva, J;Ciudad, L;Lorenzo-Mohamed, M;González-Rodríguez, M;Castellanos, B;Sotolongo-Ravelo, J;San-Segundo, L;Corchete, LA;González-Méndez, L;Martín-Sánchez, M;Mateos, MV;Ocio, EM;Garayoa, M;Paíno, T;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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