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Human CD164 Antibody

Catalog #: AF5790
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5790
AF5790-SP
Detection of Human CD164 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD164 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD164 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD164 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD164 extracellular domain
Asp24-Leu197
Accession # Q04900
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD164 antibody by Western Blot. View Larger

Detection of Human CD164 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5790) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD164 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD164

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

Entrez Gene IDs
8763 (Human); 53599 (Mouse)
Alternate Names
CD164 antigen; CD164 molecule, sialomucin; CD164; Endolyn; MGC-24; MGC-24v; MUC-24; Multi-glycosylated core protein 24; sialomucin

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Citations for Human CD164 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD164 is a host factor for lymphocytic choriomeningitis virus entry
    Authors: MJG Bakkers, A Moon-Walke, R Herlo, V Brusic, SH Stubbs, KM Hastie, EO Saphire, TL Kirchhause, SPJ Whelan
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-02;119(10):e2119676119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
    Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A
    PLoS Genet, 2015-07-21;11(7):e1005386.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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