Human CD1c/BDCA-1 Antibody

Catalog #: AF5910
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5910
AF5910-SP

Detection of Human CD1c by Western Blot.

1 Image

All CD1c/BDCA-1 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD1c/BDCA-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD1c in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1d, and rhCD1e is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD1c/BDCA‑1
Ala19-Met302 (Phe300Ser)
Accession # P29017

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human CD1c by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD1c Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD1c at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD1c/BDCA-1

CD1c (Cluster of differentiation antigen 1c) is a 316 amino acid glycoprotein with a predicted molecular weight of 43 kDa. CD1c is a member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12 kDa beta ₂‑microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316 amino acid (aa) type I transmembrane glycoprotein. It contains a 285 aa extracellular domain (ECD) (aa 18‑302) plus a 10 aa cytoplasmic tail. The ECD shows one Ig‑like domain (aa 203‑296) that associates with beta ₂‑microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa 327‑333, a second shows an eight aa substitution for aa 298‑333, and a third shows a 50 aa substitution for aa 297‑333. There appears to be no direct mouse counterpart to human CD1c.

Entrez Gene IDs

911 (Human)

Alternate Names

BDCA1; BDCA-1; c polypeptide; CD1; CD1A; CD1c antigen; CD1c molecule; CD1c; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; R7; T-cell surface glycoprotein CD1c

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CD1c/BDCA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Hypoxic human proximal tubular epithelial cells undergo ferroptosis and elicit an NLRP3 inflammasome response in CD1c+ dendritic cells
Authors: KTK Giuliani, A Grivei, P Nag, X Wang, M Rist, K Kildey, B Law, MS Ng, R Wilkinson, J Ungerer, JM Forbes, H Healy, AJ Kassianos
Cell Death & Disease, 2022-08-27;13(8):739.
Species: Human
Sample Types: Whole Tissue
Applications: IHC