What is a Biosimilar antibody?

A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody?

This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region.

Human CD20 (Research Grade Rituximab Biosimilar) Antibody

Name: Human CD20 (Research Grade Rituximab Biosimilar) Antibody
Brand: R&D Systems
SKU: MAB9575
Rating: 5 (1 reviews)

Catalog #: MAB9575
1 Citation 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB9575-100
MAB9575-SP

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Detection of CD20 in HEK293 Human cell line Transfected with Human CD20 and eGFP by Flow Cytometry

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Product Details

Citations (1)

FAQs

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD20 (Research Grade Rituximab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu2

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CD20

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD20 in HEK293 Human cell line Transfected with Human CD20 and eGFP by Flow Cytometry HEK293 Human cell line transfected with (A) Human CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CD20 (Rituximab Biosimilar) Monoclonal Antibody (Catalog # MAB9575) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9630. The biotinylated antibody (catalog number FAB9575B) can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.

Long Name

Cluster of Differentiation 20

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Alternate Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CD20 (Research Grade Rituximab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

KIR+CD8+ and NKG2A+CD8+ Tï¿½cells are distinct innate-like populations in humans
Authors: SJ Choi, JY Koh, MS Rha, IH Seo, H Lee, S Jeong, SH Park, EC Shin
Cell Reports, 2023-03-09;42(3):112236.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization

FAQs

What is a Biosimilar antibody?
- A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.
Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody?
- This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region.

View all Antibody FAQs