Human CD200 Antibody

Catalog #: MAB27243
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB27243-SP
MAB27243-100
MAB27243-500

All CD200 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD200 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD200 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse CD200 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 325531

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD200
Gln31-Gly232
Accession # P41217.3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.01 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.2-1 µg/mL of this antibody will block 50% of the binding of 2.5 ng/mL of Recombinant Human CD200 (Catalog # 2724-CD) to immobilized Recombinant Human CD200 R (Catalog # 3414-CD) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Human CD200 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CD200 Biotinylated Antibody (Catalog # BAM27242)

Standard: Recombinant Human CD200 Fc Chimera Protein, CF (Catalog # 2724-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5‑7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14‑16).

References

Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
Wright, G.J. et al. (2001) Immunology 102:173.
Shiratori, I. et al. (2005) J. Immunol. 175:4441.
Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
Fallarino, F. et al. (2004) J. Immunol. 173:3748.
Hoek, R.M. et al. (2000) Science 290:1768.
Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
Borriello, F. et al. (1997) J. Immunol. 158:4548.
Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
Hatherley, D. et al. (2005) J. Immunol. 175:2469.
Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
Cameron, C.M. et al. (2005) J. Virol. 79:6052.
Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Entrez Gene IDs

4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2

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Citation for Human CD200 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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