What is a Biosimilar antibody?

Name: Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Antibody
Brand: R&D Systems
SKU: MAB9926
Price: 449 USD

A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Antibody

Catalog #: MAB9926 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB9926-100
MAB9926-SP

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.

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Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human CD25/IL-2 R alpha based on Basiliximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Basiliximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu107

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CD25/IL-2 R alpha

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

0.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Blockade of Receptor-ligand Interaction

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IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody. In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Human Anti-Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926). Human IgG Isotype Control (Catalog # 1-001-A) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).

Flow Cytometry

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Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human PBMCs were treated with PHA (5 µg/mL for 48hr) then stained with (A) Human CD25/IL-2 R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

Basiliximab is a biosimilar directed against the extracellular domain of CD25/IL-2 R alpha. This product an be used in flow cytometry or various assay format to measure CD25/IL-2R alpha expression.

Long Name

Interleukin 2 Receptor alpha

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Alternate Names

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
- The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.
What is a Biosimilar antibody?
- A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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