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Human CD25/IL-2R alpha (Research Grade Daclizumab Biosimilar) Antibody

Catalog #: MAB9927
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9927-100
MAB9927-SP
Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.
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Human CD25/IL-2R alpha (Research Grade Daclizumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD25/IL-2 R alpha in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Daclizumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu102
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CD25/IL-2 R alpha
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD25/IL-2 Ra antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human PBMCs were treated with PHA (5 µg/mL for 24hr) then stained with (A) Human CD25/IL-2 Ra (Daclizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9927) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

Daclizumab is a biosimilar directed against the extracellular domain of CD25/IL-2 R alpha. This product can also be used in flow cytometry or various assay formats to measure CD25/IL-2 R alpha.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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Citation for Human CD25/IL-2R alpha (Research Grade Daclizumab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Antibody-guided design and identification of CD25-binding small antibody mimetics using mammalian cell surface display
    Authors: K See, T Kadonosono, K Miyamoto, T Tsubaki, Y Ota, M Katsumi, S Ryo, K Aida, M Minegishi, T Isozaki, T Kuchimaru, S Kizaka-Kon
    Scientific Reports, 2021-11-11;11(1):22098.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Functional Assay

FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.

  2. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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