Human CD25/IL-2R alpha APC-conjugated Antibody Summary
Glu22-Cys213
Accession # P01589
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2R alpha
Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
Product Datasheets
Citations for Human CD25/IL-2R alpha APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors
Authors: Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu
Oncol Lett, 2017-06-01;14(2):1363-1372.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Narcolepsy Type 1 Is Associated with a Systemic Increase and Activation of Regulatory T Cells and with a Systemic Activation of Global T Cells
Authors: M Lecendreux, G Churlaud, F Pitoiset, A Regnault, TA Tran, R Liblau, D Klatzmann, M Rosenzwajg
PLoS ONE, 2017-01-20;12(1):e0169836.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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