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Human CD27/TNFRSF7 Antibody

Catalog #: MAB382
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB382-SP
MAB382-100
MAB382-500
CD27/TNFRSF7 Inhibition of CD27 Ligand/TNFSF7-dependent Cell Proliferation and Neutralization by Human CD27/TNFRSF7 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD27/TNFRSF7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI is observed.
Source
Monoclonal Mouse IgG1 Clone # 57703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27
Thr21-Ile192
Accession # P26842
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD27/TNFRSF7 Fc Chimera (Catalog # 382-CD)
Flow Cytometry
2.5 µg/106 cells
Human whole blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CD27/TNFRSF7-induced inhibition of proliferation in mouse splenic T cells. The Neutralization Dose (ND50) is typically 0.2-0.4 µg/mL in the presence of 3 µg/mL Recombinant Human CD27/TNFRSF7 Fc Chimera, 10 µg/mL Recombinant Mouse CD27 Ligand/TNFSF7, and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization CD27/TNFRSF7 Inhibition of CD27 Ligand/TNFSF7-dependent Cell Proliferation and Neutralization by Human CD27/TNFRSF7 Antibody. View Larger

CD27/TNFRSF7 Inhibition of CD27 Ligand/TNFSF7-dependent Cell Proliferation and Neutralization by Human CD27/TNFRSF7 Antibody. In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Anti-body (Catalog # MAB484) and Recombinant Mouse CD27 Ligand (10 µg/mL, Catalog # 783-CL), Recombinant Human CD27 Fc Chimera (Catalog # 382-CD) inhibits proliferation in mouse splenic T cells in a dose-dependent manner (orange line). Under these conditions, inhibition of proliferation elicited by Recombinant Human CD27 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD27 Monoclonal Antibody (Catalog # MAB382). The ND50 is typically 0.2-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4+ compartment, it is preferentially expressed on CD45RA+ cells. In contrast, it is preferentially expressed on CD45RO+ cells in the CD8+ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide‑linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.

References
  1. Camerini, D. et al. (1991) J. Immunol. 147:3165.
  2. Loenen, W.A. et al. (1992) J. Immunol. 149:3937.
  3. Lens, S.M.A. et al. (1998) Sem. Immunol. 10:491.
Entrez Gene IDs
939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
Alternate Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Citations for Human CD27/TNFRSF7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. IL-15-secreting CAR natural killer cells directed toward the pan-cancer target CD70 eliminate both cancer cells and cancer-associated fibroblasts
    Authors: Van den Eynde, A;Gehrcken, L;Verhezen, T;Lau, HW;Hermans, C;Lambrechts, H;Flieswasser, T;Quatannens, D;Roex, G;Zwaenepoel, K;Marcq, E;Joye, P;Cardenas De La Hoz, E;Deben, C;Gasparini, A;Montay-Gruel, P;Le Compte, M;Lion, E;Lardon, F;Van Laere, S;Siozopoulou, V;Campillo-Davo, D;De Waele, J;Pauwels, P;Jacobs, J;Smits, E;Van Audenaerde, JRM;
    Journal of hematology & oncology
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
    Authors: Yoshiaki Abe, Mamiko Sakata-Yanagimoto, Manabu Fujisawa, Hiroaki Miyoshi, Yasuhito Suehara, Keiichiro Hattori et al.
    Nature Cell Biology
  3. IL-7 decreases IL-7 receptor alpha (CD127) expression and induces the shedding of CD127 by human CD8+ T cells.
    Authors: Vranjkovic A, Crawley AM, Gee K, Kumar A, Angel JB
    Int. Immunol., 2007-10-22;19(12):1329-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The immune-inflammatory response of oligodendrocytes in a murine model of preterm white matter injury: the role of TLR3 activation
    Authors: M Boccazzi, J Van Steenw, AL Schang, V Faivre, T Le Charpen, C Bokobza, Z Csaba, C Verderio, M Fumagalli, S Mani, P Gressens
    Cell Death & Disease, 2021-02-08;12(2):166.

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