Human CD27/TNFRSF7 APC-conjugated Antibody

Catalog #: FAB382A Datasheet / COA / SDS

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FAB382A

Detection of CD27/TNFRSF7 in Human PBMCs by Flow Cytometry.

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All CD27/TNFRSF7 Antibodies

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Human CD27/TNFRSF7 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI.

Source

Monoclonal Mouse IgG₁ Clone # 57703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27
Thr21-Ile192
Accession # P26842

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD27/TNFRSF7 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD27/TNFRSF7 APC-conjugated Monoclonal Antibody (Catalog # FAB382A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD27/TNFRSF7

Human CD27 is a 50-55 kDa lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes as well as NK cells, B cells, and CD4⁺ and CD8⁺ T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4⁺ compartment, it is preferentially expressed on CD45RA⁺ cells. In contrast, it is preferentially expressed on CD45RO⁺ cells in the CD8⁺ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide-linked dimer on the cell surface and as a soluble 32 kDa protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 19 aa signal, a 173 aa extracellular domain, a 21 aa transmembrane domain, and a 48 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and multiple activated leukocytes including NK cells, CD4⁺ and CD8⁺ T cells, and gamma δ T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses. Over aa 21-192, human and mouse CD27 share 60% aa sequence identity.

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Alternate Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7