Human CD3 (Research Grade Teplizumab Biosimilar) Antibody

Catalog #: MAB9929 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB9929-100
MAB9929-SP

Detection of CD3 in Human PBMCs by Flow Cytometry.

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Human CD3 (Research Grade Teplizumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human CD3 based on Teplizumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Teplizumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu113

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CD3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD3 in Human PBMCs by Flow Cytometry. Human PBMCs were stained with (A) Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929) or (B) no primary antibody, followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD3 in Human Jurkat cell line by Flow Cytometry. Human Jurkat cell line was stained with Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929, filled histogram) or Normal Human IgG control antibody (Catalog # 1-001-A, open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: CD3

Teplizumab is a biosimilar directed against the extracellular domain of CD3. This antibody can be used in flow cytometry or various assay format to measure CD3 expression.

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
- The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.
What is a Biosimilar antibody?
- A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.
What is the light chain of Human CD3 (Research Grade Teplizumab Biosimilar) Antibody, Catalog # MAB9929?
- The light chain is kappa.