Human CD30 Ligand/TNFSF8 Biotinylated Antibody

Catalog #: BAF1028 Datasheet / COA / SDS

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BAF1028

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD30 Ligand/TNFSF8 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD30 Ligand/TNFSF8 Ligand in Western blots. In this format, approximately 5% cross-reactivity with recombinant human (rh) CD27 Ligand is observed and less than 1% cross-reactivity with rhCD40 Ligand is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD30 Ligand/TNFSF8 (R&D Systems, Catalog # 1028-CL)
Gln63-Asp234
Accession # P32971

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CD30 Ligand/TNFSF8 (Catalog # 1028-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30 Ligand/TNFSF8

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4⁺/CD8⁺ thymocytes that co‑express CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a costimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30⁺ lymphomas. Human and mouse CD30 ligand cDNAs share 70% sequence homology.

References

Brunangelo, F. et al. (1995) Blood 85:1.
Gruss, H.-J. and F. Herrmann (1996) Leukemia and Lymphoma 20:397.
Chiarle, R. et al. (1999) J. Immunol. 163:194.

Entrez Gene IDs

944 (Human); 21949 (Mouse)

Alternate Names

CD153 antigen; CD153; CD30 antigen ligand; CD30 Ligand; CD30L; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8