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Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody

Catalog #: MAB9576 Datasheet / COA / SDS
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9576-100
MAB9576-SP
Detection of CD30/TNFRSF8 in Jurkat human acute T cell leukemia cell line by Flow Cytometry.
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Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD30/TNFRSF8 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Brentuximab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu8
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CD30/TNFRSF8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD30/TNFRSF8 antibody in Jurkat human acute T cell leukemia cell line antibody by Flow Cytometry. View Larger

Detection of CD30/TNFRSF8 in Jurkat human acute T cell leukemia cell line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human Anti-Human CD30/TNFRSF8 (Brentuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9576, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30/TNFRSF8

Brentuximab is a biosimilar directed against the extracellular domain of CD30. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9584. The biotinylated antibody (catalog number FAB9576B) can be used in flow cytometry or various assay format to measure CD30 expression and can be detected by conjugated streptavidin.

Entrez Gene IDs
943 (Human); 21941 (Mouse); 25069 (Rat)
Alternate Names
CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8

Product Datasheets

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FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  2. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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