Human CD38 Fluorescein-conjugated Antibody

Name: Human CD38 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB2404F
Rating: 4 (1 reviews)

Catalog #: FAB2404F 1 Review Datasheet / COA / SDS

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FAB2404F

Detection of CD38 in Whole Blood Monocytes by Flow Cytometry.

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Human CD38 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD38.

Source

Monoclonal Mouse IgG_2A Clone # 240742

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD38
Met1-Ile300
Accession # P28907

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD38 in Whole Blood Monocytes by Flow Cytometry. Whole blood monocytes were stained with Mouse Anti-Human CD38 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2404F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD38 in Whole Blood Lymphocytes by Flow Cytometry. Whole blood lymphocytes were stained with Mouse Anti-Human CD38 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2404F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD38

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)⁺ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP⁺, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5).

References

Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
Sun, L. et al. (1999) J. Cell Biol. 146:1161.
Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
Kato, I. et al. (1995) J. Biol. Chem. 270:30045.

Long Name

Cluster of Differentiation 38

Entrez Gene IDs

952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)

Alternate Names

2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD_antigen: CD38; CD38 antigen (p45); CD38 antigen; CD38 molecule; CD38; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; Cyclic ADP-ribose Hydrolase; EC 2.4.99.20; EC 3.2.2.6; EC:3.2.2.6; NAD(+) nucleosidase; T10