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Human CD39/ENTPD1 Antibody

Catalog #: MAB4397
1 Citation Datasheet / COA / SDS
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MAB4397
MAB4397-SP
Detection of CD39/ENTPD1 in Human Monocytes by Flow Cytometry.
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Human CD39/ENTPD1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD39/ENTPD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD39, recombinant human CD39L2, 3, or 4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 498403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD39/ENTPD1
Thr38-Val478
Accession # P49961
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD39/ENTPD1 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of CD39/ENTPD1 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human CD39/ENTPD1 Mono-clonal Antibody (Catalog # MAB4397, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phy-coerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. rhNTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thrombo regulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).

References
  1. Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
  2. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
  3. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
  4. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
  5. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
  6. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Entrez Gene IDs
953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)
Alternate Names
ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1

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Citation for Human CD39/ENTPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease
    Authors: F Muhammad, D Wang, A Montieth, S Lee, J Preble, CS Foster, TA Larson, K Ding, JD Dvorak, DJ Lee
    Sci Rep, 2019-11-15;9(1):16941.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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