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Human CD45 Antibody

Catalog #: MAB1430
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1430
MAB1430-SP
Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD45 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD45. This antibody recognizes all isoforms of human CD45.
Source
Monoclonal Mouse IgG1 Clone # 2D1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human peripheral blood mononuclear cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
CD45 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in CD45 knockout THP-1 cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD45 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD45 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD45 in Human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA blast using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry Detection of CD45 antibody in THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD45 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (MAB002), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Knockout Validated CD45 Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

CD45 Specificity is Shown by Flow Cytometry in Knockout Cell Line. CD45 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (MAB002) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (F0102B). No staining in the CD45 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citations for Human CD45 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates
    Authors: MJ Costa, J Kudaravall, JT Ma, WH Ho, K Delaria, C Holz, A Stauffer, AG Chunyk, Q Zong, E Blasi, B Buetow, TT Tran, K Lindquist, M Dorywalska, A Rajpal, DL Shelton, P Strop, SH Liu
    Sci Rep, 2019-02-21;9(1):2443.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019-02-01;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation
    Authors: J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li
    Nat Commun, 2019-01-25;10(1):448.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model
    Authors: JR Fan, HT Lee, W Lee, CH Lin, CY Hsu, CH Hsieh, WC Shyu
    Cell Death Dis, 2018-05-01;9(5):502.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Blockade of Tumor-Expressed PD-1 promotes lung cancer growth
    Authors: S Du, N McCall, K Park, Q Guan, P Fontina, A Ertel, T Zhan, AP Dicker, B Lu
    Oncoimmunology, 2018-01-29;7(4):e1408747.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA
    Authors: NS Harasymowi, ND Clement, A Azfer, R Burnett, DM Salter, AH Simpson
    Arthritis & rheumatology (Hoboken, N.J.), 2017-06-10;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
    Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
    Respir. Res., 2017-05-22;18(1):100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors
    Authors: CL Roberts, SS Chen, AC Murchison, RA Ogle, MP Francis, RC Ogle, PC Sachs
    Stem Cells Int, 2017-01-30;2017(0):1513281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016-10-20;2016(0):6104780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
    Authors: Keats E, Dominguez J, Grant M, Khan Z
    Stem Cells, 2014-06-01;32(6):1649-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Isolation and characterization of synovial mesenchymal stem cells.
    Authors: Harvanova D, Tothova T, Sarissky M, Amrichova J, Rosocha J
    Folia Biol. (Praha), 2011-01-01;57(3):119-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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