Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

What is a Biosimilar antibody?

A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

Catalog #: MAB9889
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB9889-100
MAB9889-SP

Detection of CD52 in human PBMC lymphocytes by Flow Cytometry.

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All CD52 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD52 based on Alemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Alemtuzumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu116

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CD52

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD52 in human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Human Anti-Human CD52 (Alemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9889, filled histogram) or no primary antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD52

Alemtuzumab is a biosimilar directed against the extracellular domain of CD52. The biotinylated antibody (catalog number FAB9889B) can be used in flow cytometry or various assay format to measure CD52 expression and can be detected by conjugated streptavidin.

Entrez Gene IDs

1043 (Human); 23833 (Mouse)

Alternate Names

Cambridge pathology 1 antigen; CAMPATH-1 antigen; CAMPATH-1; CD52 antigen (CAMPATH-1 antigen); CD52 antigen; CD52 molecule; CD52; CDW52 antigen (CAMPATH-1 antigen); CDw52; Epididymal secretory protein E5; He5; HEL-S-171mP; Human epididymis-specific protein 5

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CD52-targeted depletion by Alemtuzumab ameliorates allergic airway hyperreactivity and lung inflammation
Authors: P Shafiei-Ja, DG Helou, BP Hurrell, L Galle-Treg, E Howard, C Quach, JD Painter, M Fung, R Lo, H Allayee, O Akbari
Mucosal Immunology, 2021-03-17;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

FAQs

Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
- The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.
What is a Biosimilar antibody?
- A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

View all Antibody FAQs

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