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Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

Catalog #: MAB9889
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9889-100
MAB9889-SP
Detection of CD52 in human PBMC lymphocytes by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD52 based on Alemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Alemtuzumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu116
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CD52
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD52 antibody in human PBMC lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD52 in human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Human Anti-Human CD52 (Alemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9889, filled histogram) or no primary antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD52

Alemtuzumab is a biosimilar directed against the extracellular domain of CD52. The biotinylated antibody (catalog number FAB9889B) can be used in flow cytometry or various assay format to measure CD52 expression and can be detected by conjugated streptavidin.

Entrez Gene IDs
1043 (Human); 23833 (Mouse)
Alternate Names
Cambridge pathology 1 antigen; CAMPATH-1 antigen; CAMPATH-1; CD52 antigen (CAMPATH-1 antigen); CD52 antigen; CD52 molecule; CD52; CDW52 antigen (CAMPATH-1 antigen); CDw52; Epididymal secretory protein E5; He5; HEL-S-171mP; Human epididymis-specific protein 5

Product Datasheets

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Citation for Human CD52 (Research Grade Alemtuzumab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD52-targeted depletion by Alemtuzumab ameliorates allergic airway hyperreactivity and lung inflammation
    Authors: P Shafiei-Ja, DG Helou, BP Hurrell, L Galle-Treg, E Howard, C Quach, JD Painter, M Fung, R Lo, H Allayee, O Akbari
    Mucosal Immunology, 2021-03-17;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.

  2. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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