Human CD83 Fluorescein-conjugated Antibody

Catalog #: FAB1774F
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FAB1774F

Detection of CD83 in Human CD14+Monocyte-derived Dendritic Cells by Flow Cytometry.

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Human CD83 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD83.

Source

Monoclonal Mouse IgG₁ Clone # HB15e

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD83 in Human CD14⁺Monocyte-derived Dendritic Cells by Flow Cytometry. Human CD14⁺monocyte-derived dendritic cells were stained with Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD83

Human CD83 is a 40‑50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8⁺ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4⁺ T cell phenotype (17).

References

Zhou, L-J. et al. (1992) J. Immunol. 149:735.
Kozlow, E.J. et al. (1993) Blood 81:454.
Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
Hock, B.D. et al. (2001) Int. Immunol. 13:959.
Dudziak, D. et al. (2005) J. Immunol. 174:6672.
Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
Yamashiro, S. et al. (2000) Blood 96:3958.
Cao, W. et al. (2005) Biochem. J. 385:85.
Scholler, N. et al. (2001) J. Immunol. 166:3865.
Scholler, N. et al. (2002) J. Immunol. 168:2599.
Hirano, N. et al. (2006) Blood 107:1528.
Kotzor, N. et al. (2004) Immunobiology 209:129.
Zinser, E. et al. (2006) Immunobiology 211:449.
Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Entrez Gene IDs

9308 (Human); 12522 (Mouse)

Alternate Names

B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83

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Citation for Human CD83 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
Authors: KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge
Front Microbiol, 2017-03-07;8(0):321.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry