Human CD94 Antibody

Catalog #: MAB10581 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10581-100
MAB10581-SP

Detection of CD94 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD94 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD94 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1032011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human CD94
Lys32-Ile179
Accession # Q13241.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human Blood Lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD94 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD94 Monoclonal Antibody (Catalog # MAB10581) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by anti-Mouse IgG Allophycocyanin-conjugated secondary antibody (F0101B) and Mouse Anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8⁺ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References

Chester, C. et al. (2015) Front. Immunol. 6:601.
Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
Lieto, L.D. et al. (2006) Genes Immun. 7:36.
Yu, J. et al. (2010) Blood 115:274.
Phillips, J.H. et al. (1996) Immunity 5:163.
Lazetic, S. et al. (1996) J. Immunol. 157:4741.
Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
Braud, V.M. et al. (1998) Nature 391:795.
Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
Houchins, J.P. et al. (1997) J. Immunol. 158:3603.

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Alternate Names

CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor