Human CD94 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1058P
Detection of CD94 in Human PBMCs by Flow Cytometry.
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Human CD94 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.
Source
Monoclonal Mouse IgG1 Clone # 131412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD94 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD94 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD94 PE-conjugated Monoclonal Antibody (Catalog # FAB1058P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD94

CD94, also known as KLAD1, is a 30 kDa type II transmembrane protein with an extracellular C-type lectin domain. It is expressed by natural killer (NK) cells and a subset of CD8+ T cells, with cellular activation resulting in increased cell surface expression. Although CD94 can occur as a non-signaling homodimer, functional activity occurs when CD94 exists as a heterodimer with an NKG2 family member. A CD94/NKG2A heterodimer delivers an inhibitory signal to the expressing cell, whereas, a CD94/NKG2C heterodimer associates with the DAP12 adapter protein and delivers an activating signal. Both heterodimeric complexes recognize HLA-E with an associated peptide derived from the signal peptide of other HLA proteins.

Entrez Gene IDs
3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)
Alternate Names
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor

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