Human CDO Biotinylated Antibody

Catalog #: BAF4384 Datasheet / COA / SDS

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BAF4384

All CDO Antibodies

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Human CDO Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CDO in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse CDO is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CDO
Asp26-Pro943 Leu669Ile)
Accession # NP_058648

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CDO (Catalog # 4384-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDO

CDO (CAM-related/down‑regulated by oncogenes, also CDON; pronounced “kid-oh”) is a 190 kDa member of the Immunoglubulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human CDO is a type I transmembrane (TM) glycoprotein. It is synthesized as a 1287 amino acid (aa) precursor that contains a 25 aa signal sequence, a 938 aa extracellular domain (ECD), a 21 aa TM segment and a 303 aa cytoplasmic region (1, 2). The ECD contains five C2‑type Ig-like domains, followed by three FN type III repeats. The first FN repeat (aa 577‑673) is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat (aa 826‑923) binds SHH (3, 4). The intracellular region is believed to signal through various bHLH transcription factors (2). One alternate splice form is reported that shows a deletion of aa 1212‑1234 in the cytoplasmic tail. The ECD of human CDO is 85% aa identical to mouse CDO ECD. CDO is found on muscle precursor and neural progenitor cells of the embryo (5, 6). It likely promotes muscle differentiation, and contributes to axon guidance and neuronal patterning (2, 7, 8, 9). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (2). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly‑defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (7, 8, 10).

References

Kang, J.S. et al. (1997) J. Cell Biol. 138:203.
Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
Yao, S. et al. (2006) Cell 125:343.
Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989.
Kang, J-S. et al. (2002) EMBO J. 21:114.
Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
Okada, A. et al. (2006) Nature 444:369.
Allen, B.L. et al. (2007) Genes Dev. 21:1244.
Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
Tenzen, T. et al. (2006) Dev. Cell 10:647.

Long Name

Cell Adhesion Molecule-related/Down-regulated by Oncogenes

Entrez Gene IDs

50937 (Human); 57810 (Mouse)

Alternate Names

CDO; Cdon homolog (mouse); CDON; CDOORCAM; cell adhesion molecule-related/down-regulated by oncogenes; MGC111524; ORCAM; surface glycoprotein, Ig superfamily member