Human Choline Acetyltransferase/ChAT Antibody

Catalog #: MAB3447
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3447
MAB3447-SP

All Choline Acetyltransferase/ChAT Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Choline Acetyltransferase/ChAT Antibody Summary

Species Reactivity

Human

Specificity

Detects human Choline Acetyltransferase/ChAT in direct ELISAs and Western blots. Recognizes both ChAT isoform 1 (ChAT-69) and isoform 2 (ChAT-82).

Source

Monoclonal Mouse IgG₁ Clone # 334008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Choline Acetyltransferase/ChAT isoform 1
Ala2-Pro630
Accession # NP_066266

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Choline Acetyltransferase/ChAT

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Choline Acetyltransferase/ChAT

ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinogenic neuron communication. It serves as a marker for functional cholinogenic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N‑terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.

Entrez Gene IDs

1103 (Human); 12647 (Mouse); 290567 (Rat)

Alternate Names

acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; Choline Acetyltransferase; choline O-acetyltransferase; CMS1A; CMS1A2; EC 2.3.1; EC 2.3.1.6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Neurotransmitters, Neuroactive Molecules and Associated Enzymes

Citation for Human Choline Acetyltransferase/ChAT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1