Human CHRM2 Antibody

Catalog #: MAB6377 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6377
MAB6377-SP

Detection of CHRM2 in HEK293 Human Cell Line transfected with Human CHRM2 and eGFP by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CHRM2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CHRM2 in immunohistochemistry.

Source

Monoclonal Mouse IgG_2B Clone # 585713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CHRM2
Accession # P08172

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line transfected with Human CHRM2 and eGFP

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human heart

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CHRM2 in HEK293 Human Cell Line transfected with Human CHRM2 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human CHRM2 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human CHRM2 monoclonal antibody (Catalog # MAB6377) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM2

Muscarinic acetylcholine receptor M2 (CHRM2) is a 60 kDa 7-transmembrane glycoprotein that is predominantly expressed in the heart and central nervous system. CHRM2 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Polymorphisms of CHRM2 are associated with alcohol dependence, depression, bipolar disorder, and recovery of heart function after myocardial infarction. Human CHRM2 shares 96% aa sequence identity with mouse and rat CHRM2.

Long Name

Cholinergic Receptor Muscarinic 2

Entrez Gene IDs

1129 (Human)

Alternate Names

7TM receptor; cholinergic receptor, muscarinic 2; CHRM2; FLJ43243; HM2; mAChR M2; MGC120006; MGC120007; muscarinic acetylcholine receptor M2; muscarinic M2 receptor