Human Chymase/CMA1 Antibody

Catalog #: MAB40991 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB40991-100
MAB40991-SP

Human Chymase/CMA1 ELISA Standard Curve.

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All Chymase/CMA1 Antibodies

Product Details

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chymase/CMA1 Antibody Summary

Species Reactivity

Human

Specificity

detects human Chymase/CMA1 in drirect ELISAs.

Source

Monoclonal Mouse IgG₃ Clone # 422804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1
Gly20-Asn247
Accession # P23946

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Chymase/CMA1 DuoSet ELISA Kit (Catalog # DY4099-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Chymase/CMA1 ELISA Standard Curve. Recombinant Human Chymase/CMA1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB40991) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (MAB4099) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chymase/CMA1

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2‑residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References

Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.

Entrez Gene IDs

1215 (Human); 17228 (Mouse); 25627 (Rat)

Alternate Names

Alpha-chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Chymase; chymase, heart; chymase, mast cell; CMA1; CYH; CYM; EC 3.4.21; EC 3.4.21.39; Mast cell protease I; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891