Human cIAP-1/HIAP-2 Antibody

Catalog #: MAB8181 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8181
MAB8181-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human cIAP-1/HIAP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human cIAP-1/HIAP-2 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human cIAP-2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 681724

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human cIAP-1/HIAP-2
His2-Ser618
Accession # Q13490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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cIAP‑1/HIAP‑2 in Human Prostate. cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB8181) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: cIAP-1/HIAP-2

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.

References

Roy, N. et al. (1997) EMBO J. 23:6914.
Deveraux, Q. et al. (1997) Nature 388:300.
Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
Herrera, B. et al. (2002) FEBS Letters 520:93.

Long Name

Cellular Inhibitor of Apoptosis Protein 1

Entrez Gene IDs

329 (Human); 11797 (Mouse)

Alternate Names

API1Hiap-2; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing protein 2; BIRC2; cIAP1; c-IAP1; cIAP-1; hIAP2; HIAP-2; IAP homolog B; IAP2; IAP-2; Inhibitor of apoptosis protein 2; MIHB; MIHBHIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RNF48hiap-2; TNFR2-TRAF-signaling complex protein 2