Human cIAP-1/HIAP-2 Antibody Summary
His2-Ser618
Accession # Q13490
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human cIAP‑1/HIAP‑2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, lystates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line were included. A specific band for cIAP-1/HIAP-2 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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cIAP‑1/HIAP‑2 in Human Lymph Node. cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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cIAP‑1/HIAP‑2 in Human Lymphoma. cIAP-1/HIAP-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human cIAP‑1/HIAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for cIAP-1/HIAP-2 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Human cIAP‑1/HIAP‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for cIAP-1/HIAP-2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Specificity of Human cIAP‑1/HIAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for cIAP-1/HIAP-2 at approximately 66 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Goat Anti-Human cIAP-1/HIAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) was used at 5 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human cIAP-1/HIAP-2 by Western Blot IAP expression in glioblastoma cell lines. Expression levels of cIAP1, cIAP2, XIAP and ML-IAP were analyzed by western blotting and quantified in U87MG and GL261 adherent GBM cell lines, and in GBM6 and GBM9 spheres. Expression level of beta -actin served as loading control. The four GBM cell lines expressed heterogeneously cIAP1, cIAP2, XIAP and ML-IAP. A representative experiment of four experiments is shown. Quantification was performed using ImageJ software (National Institutes of Health, Bethesda, MD, USA) and data presented were normalized to beta -actin expression Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27490930), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human cIAP-1/HIAP-2 by Western Blot cIAP-1 and cIAP-2 are downregulated in cholangiocytes of PSC patients. (a) Representative images of liver sections stained for cIAP-1 (left panel) and cIAP-2 (right panel) in patients with normal, NASH and PSC (stage IV) liver histology. Photomicrographs of small bile ducts (SBD) and large bile ducts (LBD) taken at × 20 magnification. The arrows point to the bile ducts. (b) Histological scoring for cIAP-1 in normal (5, 31), NASH (12, 120) and PSC (16, 131) patients and for cIAP-2 in normal (5, 31), NASH (12, 118) and PSC (16, 123) patients. Numbers in parentheses indicate total number of patients and total number of small and large bile ducts evaluated, respectively. Grade 0=no protein expression; grade 3=high protein expression. **P<0.01, ***P<0.001. (c) Representative images of liver sections stained for TWEAK (left panel) and Fn14 (right panel) from patients with normal or PSC (stage IV) liver histology. Photomicrographs of small bile ducts and large bile ducts taken at × 40 and × 20 magnification, respectively. The arrows point to the bile ducts. (d) Immunoblot analysis showing expression of cIAP-1, cIAP-2 and actin (loading control) in H69 cells treated with human recombinant TWEAK (100 ng/ml) for the indicated times Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis2016459), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human cIAP-1/HIAP-2 by Immunocytochemistry/Immunofluorescence Prognostic value of cIAP1, cIAP2, XIAP and ML-IAP protein expression in human glioblastomas (cohorts 1 and 2). (a) cIAP1-, cIAP2-, XIAP- and ML-IAP-positive stainings in GBM. IAPs were heterogeneously expressed by tumor cells in GBM samples (Table 1). Stainings were diffused with a stronger punctuated positivity into the cytoplasm. Black arrows highlight cIAP2-positive nuclei. Scale bars, 50 μm. (b) Correlation of ML-IAP protein expression with PFS and OS in cohort 1. The cutoff was 35% and was determined by performing a ROC curve. ML-IAP expression of ⩾35% was correlated with a poor prognosis. (c) Correlation of ML-IAP protein expression with PFS and OS in cohort 2. The cutoff was the same as that for cohort 1 analysis (35%). ML-IAP expression of ⩾35% was correlated with a poor prognosis Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27490930), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human cIAP-1/HIAP-2 by Western Blot Apoptosis and IAP expression upon SMAC mimetic GDC-0152 treatment in glioblastoma cell lines. (a) Apoptosis (SubG0/G1) of DMSO control and GDC-0152-treated cells was determined by flow cytometry of propidium iodide-stained nuclei and percentage of apoptosis is shown. U87MG and GL261 cell lines were treated for 72 h and GBM6 and GBM9 cell lines were treated for 8 days at the indicated concentrations. At these respective time points, percentage of U87MG cells dead by apoptosis, percentage of GL261 cells, percentage of GBM6 cells and percentage of GBM9 cells. Data are expressed as mean+S.E.M. Three independent experiments were performed for the GL261 cell lines and five for the U87MG, GBM6 and GBM9 cell lines. *P<0.05; **P<0.01; ***P<0.005. (b) Expression levels of cIAP1, cIAP2, XIAP and ML-IAP were analyzed by western blotting. Cell lines were treated with 1 μM of GDC-0152. U87MG and GL261 were treated for 72 h and GBM6 and GBM9 cell lines for 8 days. In all GBM cell lines GDC-0152 decreased IAP expression. Expression level of beta -actin served as loading control. A representative experiment of three experiments is shown Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27490930), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-1/HIAP-2
cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Herrera, B. et al. (2002) FEBS Letters 520:93.
Product Datasheets
Citations for Human cIAP-1/HIAP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The SMAC mimetic BV6 sensitizes colorectal cancer cells to ionizing radiation by interfering with DNA repair processes and enhancing apoptosis.
Authors: Hehlgans S, Oppermann J, Reichert S et al.
Radiat Oncol
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Identification of F-box only protein 7 as a negative regulator of NF-kappaB signalling
Authors: Hendrik J. Kuiken, David A. Egan, Heike Laman, Rene Bernards, Roderick L. Beijersbergen, Annette M. Dirac
Journal of Cellular and Molecular Medicine
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Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152
Authors: Tchoghandjian A, Souberan A, Tabouret E et al.
Cell Death Dis
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A Nucleolar Stress–Specific p53–miR-101 Molecular Circuit Functions as an Intrinsic Tumor-Suppressor Network
Authors: Yuko Fujiwara, Motonobu Saito, Ana I. Robles, Momoyo Nishida, Fumitaka Takeshita, Masatoshi Watanabe et al.
EBioMedicine
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Pharmacological Activation of Non-canonical NF-kappa B Signaling Activates Latent HIV-1 Reservoirs In Vivo
Authors: Lars Pache, Matthew D. Marsden, Peter Teriete, Alex J. Portillo, Dominik Heimann, Jocelyn T. Kim et al.
Cell Reports Medicine
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SNIPER(TACC3) induces cytoplasmic vacuolization and sensitizes cancer cells to Bortezomib
Authors: Nobumichi Ohoka, Katsunori Nagai, Norihito Shibata, Takayuki Hattori, Hiroshi Nara, Nobuo Cho et al.
Cancer Science
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NF-kB functions as a molecular link between tumor cells and Th1/Tc1 T cells in the tumor microenvironment to exert radiation-mediated tumor suppression.
Authors: Simon PS, Bardhan K, Chen MR et al.
Oncotarget.
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Lycopene Inhibits Reactive Oxygen Species-Mediated NF-kappa B Signaling and Induces Apoptosis in Pancreatic Cancer Cells
Authors: Yoonseon Jeong, Joo Weon Lim, Hyeyoung Kim
Nutrients
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Different Degradation Mechanisms of Inhibitor of Apoptosis Proteins (IAPs) by the Specific and Nongenetic IAP-Dependent Protein Eraser (SNIPER)
Authors: Nobumichi Ohoka, Osamu Ujikawa, Kenichiro Shimokawa, Tomoya Sameshima, Norihito Shibata, Takayuki Hattori et al.
Chemical and Pharmaceutical Bulletin
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NF-kappaB inhibition improves the sensitivity of human glioblastoma cells to 5-aminolevulinic acid-based photodynamic therapy
Authors: Isabelle Coupienne, Sébastien Bontems, Michael Dewaele, Noemi Rubio, Yvette Habraken, Simone Fulda et al.
Biochemical Pharmacology
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Molecular basis for nuclear accumulation and targeting of the inhibitor of apoptosis BIRC2
Authors: Tencer, AH;Yu, Y;Causse, SZ;Campbell, GR;Klein, BJ;Xuan, H;Cartier, J;Miles, MA;Gaurav, N;Zadoroznyj, A;Holt, TA;Wen, H;Hawkins, CJ;Spector, SA;Dubrez, L;Shi, X;Kutateladze, TG;
Nature structural & molecular biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation
Authors: EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin
Cell Reports, 2023-01-16;0(0):111965.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
cIAP1/TRAF2 interplay promotes tumor growth through the activation of STAT3
Authors: B Dumétier, A Zadoroznyj, J Berthelet, S Causse, J Allègre, P Bourgeois, F Cattin, C Racoeur, C Paul, C Garrido, L Dubrez
Oncogene, 2022-11-18;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
cIAP1-based degraders induce degradation via branched ubiquitin architectures
Authors: Y Akizuki, M Morita, Y Mori, A Kaiho-Soma, S Dixit, A Endo, M Shimogawa, G Hayashi, M Naito, A Okamoto, K Tanaka, Y Saeki, F Ohtake
Nature Chemical Biology, 2022-10-31;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Induction of interferon-&beta and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells
Authors: V Granqvist, C Holmgren, C Larsson
PLoS ONE, 2021-03-26;16(3):e0248175.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas
Authors: Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen
Scientific Reports, 2021-03-18;11(1):6392.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mouse models of neutropenia reveal progenitor-stage-specific defects
Authors: DE Muench, A Olsson, K Ferchen, G Pham, RA Serafin, S Chutipongt, P Dwivedi, B Song, S Hay, K Chetal, LR Trump-Durb, J Mookerjee-, K Zhang, JC Yu, C Lutzko, KC Myers, KL Nazor, KD Greis, DJ Kappes, SS Way, N Salomonis, HL Grimes
Nature, 2020-04-22;582(7810):109-114.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Systemic HIV and SIV latency reversal via non-canonical NF-kappaB signalling in vivo
Authors: CC Nixon, M Mavigner, GC Sampey, AD Brooks, RA Spagnuolo, DM Irlbeck, C Mattingly, PT Ho, N Schoof, CG Cammon, GK Tharp, M Kanke, Z Wang, RA Cleary, AA Upadhyay, C De, SR Wills, SD Falcinelli, C Galardi, H Walum, NJ Schramm, J Deutsch, JD Lifson, CM Fennessey, BF Keele, S Jean, S Maguire, B Liao, EP Browne, RG Ferris, JH Brehm, D Favre, TH Vanderford, SE Bosinger, CD Jones, JP Routy, NM Archin, DM Margolis, A Wahl, RM Dunham, G Silvestri, A Chahroudi, JV Garcia
Nature, 2020-01-22;578(7793):160-165.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways
Authors: J Dittmann, T Haydn, P Metzger, GA Ward, M Boerries, M Vogler, S Fulda
Cell Death Differ., 2019-12-12;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The LQB-223 Compound Modulates Antiapoptotic Proteins and Impairs Breast Cancer Cell Growth and Migration
Authors: LGT Lemos, GMDC Longo, BDS Mendonça, MC Robaina, MCM Brum, CA Cirilo, ERP Gimba, PRR Costa, CD Buarque, G Nestal de, RC Maia
Int J Mol Sci, 2019-10-12;20(20):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes
Authors: S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang
Cell Rep, 2019-02-19;26(8):2194-2211.e6.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot -
E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding
Authors: J Allègre, J Cartier, V Glorian, N Droin, B Dumetier, C Kayaci, J Berthelet, S Gemble, C Vuillier, L Maillet, C Garrido, L Dubrez
PLoS ONE, 2018-10-25;13(10):e0206253.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Pharmacological difference between degrader and inhibitor against oncogenic BCR-ABL kinase
Authors: N Shibata, K Shimokawa, K Nagai, N Ohoka, T Hattori, N Miyamoto, O Ujikawa, T Sameshima, H Nara, N Cho, M Naito
Sci Rep, 2018-09-10;8(1):13549.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth
Authors: GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari
Mol. Cancer Ther., 2018-04-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Derivatization of inhibitor of apoptosis protein (IAP) ligands yields improved inducers of estrogen receptor ? degradation
Authors: N Ohoka, Y Morita, K Nagai, K Shimokawa, O Ujikawa, I Fujimori, M Ito, Y Hayase, K Okuhira, N Shibata, T Hattori, T Sameshima, O Sano, R Koyama, Y Imaeda, H Nara, N Cho, M Naito
J. Biol. Chem., 2018-03-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment
Authors: A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka
Cell Death Differ., 2017-11-03;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hepatitis C Virus Infection Increases c-Jun N-Terminal Kinase (JNK) Phosphorylation and Accentuates Hepatocyte Lipoapoptosis
Authors: H Takaki, Y Akazawa, Y Kido, M Morishita, T Honda, H Shibata, S Miuma, H Miyaaki, N Taura, H Kondo, K Nakao
Med. Sci. Monit., 2017-09-21;23(0):4526-4532.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
Cancer Med, 2017-08-10;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Molluscum Contagiosum Virus MC159 abrogates cIAP1-NEMO interactions and inhibits NEMO polyubiquitination
Authors: S Biswas, JL Shisler
J. Virol., 2017-07-12;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development of protein degradation inducers of oncogenic BCR-ABL protein by conjugation of ABL kinase inhibitors and IAP ligands
Authors: N Shibata, N Miyamoto, K Nagai, K Shimokawa, T Sameshima, N Ohoka, T Hattori, Y Imaeda, H Nara, N Cho, M Naito
Cancer Sci., 2017-06-19;108(8):1657-1666.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017-05-25;8(5):e2816.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Immunoprecipitation, Western Blot -
NCTD promotes Birinapant-mediated anticancer activity in breast cancer cells by downregulation of c-FLIP
Authors: L Zhao, G Yang, H Bai, M Zhang, D Mou
Oncotarget, 2017-04-18;8(16):26886-26895.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
Authors: ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores
Cell Death Dis, 2017-01-05;8(1):e2535.
Species: Human, Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Targeted degradation of proteins localized in subcellular compartments by hybrid small molecules
Authors: Keiichiro Okuhira
Mol. Pharmacol, 2016-12-13;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival
Authors: L Hernandez, MK Kim, AM Noonan, E Sagher, H Kohlhammer, G Wright, LT Lyle, PS Steeg, M Anver, DD Bowtell, CM Annunziata
Cell Death Discov, 2015-12-14;1(0):15053.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics.
Authors: Lee E, Seong D, Seo J, Jeong M, Lee H, Song J
Cell Death Differ, 2015-01-23;22(9):1463-76.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
Anticancer Res, 2014-12-01;34(12):7007-20.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death.
Authors: Schoeneberger H, Belz K, Schenk B, Fulda S
Oncogene, 2014-11-10;34(31):4032-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
Authors: Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J, Zhang X, Dai S, Sun X
J Hematol Oncol, 2014-08-20;7(0):62.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders.
Authors: Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T
Int J Oncol, 2014-04-28;45(1):419-25.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, Gregory
PLoS ONE, 2014-03-14;9(3):e92124.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
NF-kappaB inducing kinase, a central signaling component of the non-canonical pathway of NF-kappaB, contributes to ovarian cancer progression.
Authors: Uno M, Saitoh Y, Mochida K, Tsuruyama E, Kiyono T, Imoto I, Inazawa J, Yuasa Y, Kubota T, Yamaoka S
PLoS ONE, 2014-02-12;9(2):e88347.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway.
Authors: Wang Q, Chen W, Bai L, Chen W, Padilla M, Lin A, Shi S, Wang X, Lin Y
J Biol Chem, 2014-01-14;289(9):5654-63.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
FASEB J, 2013-12-26;28(4):1938-46.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
Authors: Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M
Clin Cancer Res, 2013-02-12;19(7):1784-94.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Characterization of ML-IAP protein stability and physiological role in vivo.
Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
Biochem J, 2012-11-01;447(3):427-36.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
PLoS ONE, 2012-09-20;7(9):e45492.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration.
Authors: Oberoi-Khanuja, Tripat K, Karreman, Christia, Larisch, Sarit, Rapp, Ulf R, Rajalingam, Krishnar
J Biol Chem, 2012-06-18;287(34):28445-55.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways.
Clin. Cancer Res., 2012-05-02;18(12):3316-27.
Species: Human
Sample Types: Cell Lysates
Applications: Flow Cytometry, Western Blot -
Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-kappaB-dependent manner.
Authors: Wagner L, Marschall V, Karl S, Cristofanon S, Zobel K, Deshayes K, Vucic D, Debatin K, Fulda S
Oncogene, 2012-04-02;32(8):988-97.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis.
Authors: Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S
Leukemia, 2011-12-16;26(5):1020-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8.
Authors: Ikner A, Ashkenazi A
J. Biol. Chem., 2011-04-27;286(24):21546-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade.
Authors: Reddy RM, Yeow WS, Chua A, Nguyen DM, Baras A, Ziauddin MF, Shamimi-Noori SM, Maxhimer JB, Schrump DS
Apoptosis, 2007-01-01;12(1):55-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases.
Authors: Balasubramanian S, Mani S, Shiraishi H, Johnston RK, Yamane K, Willey CD, Cooper G, Tuxworth WJ, Kuppuswamy D
J. Mol. Cell. Cardiol., 2006-08-22;41(4):669-79.
Species: Feline
Sample Types: Tissue Homogenates
Applications: Western Blot -
cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.
Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC
Cancer Res., 2005-01-01;65(1):210-8.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications.
Authors: Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC
Oncogene, 2002-08-22;21(37):5673-83.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-kappa B signaling in activated T cells
Authors: Hongli Yin, Ozge Karayel, Ying-Yin Chao, Thomas Seeholzer, Isabel Hamp, Oliver Plettenburg et al.
Cellular and Molecular Life Sciences
-
A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation
Authors: Zhijun Liu, Himani Nailwal, Jonah Rector, Masmudur M. Rahman, Richard Sam, Grant McFadden et al.
Immunity
-
Estrogen Promotes Breast Cancer Cell Survival in an Inhibitor of Apoptosis (IAP)-dependent Manner
Authors: Adina Stanculescu, Leslie A. Bembinster, Kristina Borgen, Anna Bergamaschi, Elizabeth Wiley, Jonna Frasor
Hormones and Cancer
-
MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen
Authors: Katrin E. Tagscherer, Anne Fassl, Tabea Sinkovic, Jutta Richter, Sabrina Schecher, Stephan Macher-Goeppinger et al.
Cancer Cell International
-
Cancer cell death induced by novel small molecules degrading the TACC3 protein via the ubiquitin–proteasome pathway
Authors: N Ohoka, K Nagai, T Hattori, K Okuhira, N Shibata, N Cho et al.
Cell Death & Disease
-
Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-a-dependent necroptosis driven by RIP1 kinase and NF-kB.
Authors: Metzig MO, Fuchs D, Tagscherer KE, Grone HJ.
Oncogene.
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Dual Antagonist of cIAP/XIAP ASTX660 Sensitizes HPV(−) and HPV(+) Head and Neck Cancers To TNF alpha, TRAIL, and Radiation Therapy
Authors: Roy Xiao, Yi An, Wenda Ye, Adeeb Derakhshan, Hui Cheng, Xinping Yang et al.
Clinical Cancer Research
-
Cellular Inhibitor of Apoptosis 1 (cIAP-1) Degradation by Caspase 8 During TNF-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis.
Authors: Guicciardi ME, Mott JL, Bronk SF et al.
Exp Cell Res.
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Induction of Breast Cancer Cell Apoptosis by TRAIL and Smac Mimetics: Involvement of RIP1 and cFLIP
Authors: Christian Holmgren, Ellen Sunström Thörnberg, Victoria Granqvist, Christer Larsson
Current Issues in Molecular Biology
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Intrinsic and chemo-sensitizing activity of SMAC-mimetics on high-risk childhood acute lymphoblastic leukemia
Authors: M Schirmer, L Trentin, M Queudeville, F Seyfried, S Demir, E Tausch et al.
Cell Death & Disease
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Expression of hcIAP-1/HIAP-2 in MCF-7 cells untreated (A) and after treatment with 10 uM Paclitaxel for 4 hours (B) using Goat anti-Human cIAP-1/HIAP-2 antibody (#AF8181) at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.
