Human cIAP-2/HIAP-1 Antibody Summary
Asn2-Ser604
Accession # U45878
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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cIAP-2/HIAP-1 in Human Colon Tissue. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon tissue using Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to colon glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for cIAP-2/HIAP-1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
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Detection of Human cIAP‑2/HIAP‑1 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP-2/HIAP-1 at approximately 70 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-2/HIAP-1
cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
- Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
Product Datasheets
Citations for Human cIAP-2/HIAP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas
Authors: Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen
Scientific Reports, 2021-03-18;11(1):6392.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes
Authors: S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang
Cell Rep, 2019-02-19;26(8):2194-2211.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth
Authors: GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari
Mol. Cancer Ther., 2018-04-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
Cancer Med, 2017-08-10;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
Molecules, 2016-07-30;21(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics.
Authors: Lee E, Seong D, Seo J, Jeong M, Lee H, Song J
Cell Death Differ, 2015-01-23;22(9):1463-76.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
Anticancer Res, 2014-12-01;34(12):7007-20.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
Authors: Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M
Clin Cancer Res, 2013-02-12;19(7):1784-94.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
PLoS ONE, 2012-09-20;7(9):e45492.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.
Authors: McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR
Exp. Cell Res., 2005-01-01;302(1):69-82.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.
Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC
Cancer Res., 2005-01-01;65(1):210-8.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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Western blot analysis of Human cIAP-2/HIAP-1 in MDA-MB-231 cells untreated (A) and treated with 10 uM Paclitaxel for 4 hours (B). Goat anti-Human cIAP-2/HIAP-1antibody (#AF8171) was used at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.
