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Human cIAP-2/HIAP-1 Antibody

Catalog #: AF8171
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8171
AF8171-SP
cIAP-2/HIAP-1 in Human Colon Tissue.
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human cIAP-2/HIAP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human cIAP‑2/HIAP‑1 in Western blots. In Western blots, less than 1% cross-reactivity with cIAP-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human cIAP‑2/HIAP‑1
Asn2-Ser604
Accession # U45878
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry cIAP-2/HIAP-1 antibody in Human Colon Tissue by Immunohistochemistry (IHC-P). View Larger

cIAP-2/HIAP-1 in Human Colon Tissue. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon tissue using Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to colon glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Human cIAP-2/HIAP-1 antibody by Western Blot. View Larger

Detection of Human cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for cIAP-2/HIAP-1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Simple Western Detection of Human cIAP-2/HIAP-1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human cIAP‑2/HIAP‑1 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP-2/HIAP-1 at approximately 70 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: cIAP-2/HIAP-1

cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.

References
  1. Roy, N. et al. (1997) EMBO J. 23:6914.
  2. Deveraux, Q. et al. (1997) Nature 388:300.
  3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
  4. Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
  5. Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
Entrez Gene IDs
330 (Human)
Alternate Names
AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; cIAP2; c-IAP2; cIAP-2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49

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Citations for Human cIAP-2/HIAP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Human 3D ovarian cancer models reveal malignant cell intrinsic and extrinsic factors that influence CAR T cell activity
    Authors: Joy, JD;Malacrida, B;Laforets, F;Kotantaki, P;Maniati, E;Manchanda, R;Annibaldi, A;Hopkins, S;Garrobo-Calleja, I;Gautrot, J;Balkwill, FR;
    Cancer research
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas
    Authors: Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen
    Scientific Reports, 2021-03-18;11(1):6392.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes
    Authors: S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang
    Cell Rep, 2019-02-19;26(8):2194-2211.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth
    Authors: GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari
    Mol. Cancer Ther., 2018-04-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
    Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
    Cancer Med, 2017-08-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016-07-30;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics.
    Authors: Lee E, Seong D, Seo J, Jeong M, Lee H, Song J
    Cell Death Differ, 2015-01-23;22(9):1463-76.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
    Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
    Anticancer Res, 2014-12-01;34(12):7007-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
    Authors: Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M
    Clin Cancer Res, 2013-02-12;19(7):1784-94.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
    Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
    PLoS ONE, 2012-09-20;7(9):e45492.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.
    Authors: McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR
    Exp. Cell Res., 2005-01-01;302(1):69-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.
    Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC
    Cancer Res., 2005-01-01;65(1):210-8.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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Human cIAP-2/HIAP-1 Antibody
By Anonymous on 03/27/2017
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Western blot analysis of Human cIAP-2/HIAP-1 in MDA-MB-231 cells untreated (A) and treated with 10 uM Paclitaxel for 4 hours (B). Goat anti-Human cIAP-2/HIAP-1antibody (#AF8171) was used at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.

Western Blot Human cIAP-2/HIAP-1 Antibody AF8171