Human Claudin-19 Antibody

Catalog #: MAB6970
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6970
MAB6970-SP

Claudin-19 in Human Kidney Cancer Tissue.

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All Claudin-19 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Claudin-19 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 693202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-19
Accession # Q8N6F1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Claudin-19 in Human Kidney Cancer Tissue. Claudin-19 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissuer using Mouse Anti-Human Claudin-19 Monoclonal Antibody (Catalog # MAB6970) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-19

Claudin-19 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-19 is expressed by renal tubular epithelial cells, retinal pigment epithelial cells, and Schwann cells. It interacts with Claudin-16 to form cation-selective paracellular channels in the thick ascending loop of the kidney. Mutations of Claudin-19 are associated with renal disorders of magnesium and calcium reabsorption. Human Claudin-19 shares 95% aa sequence identity with mouse and rat Claudin-19. An alternate splice form of human Claudin-19 has a deletion within the C-terminal cytoplasmic region.

Entrez Gene IDs

149461 (Human); 242653 (Mouse); 298487 (Rat)

Alternate Names

claudin 19; Claudin19; Claudin-19; CLDN19

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Claudin-19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients
Authors: Karema-Jokinen, V;Koskela, A;Hytti, M;Hongisto, H;Viheri�l�, T;Liukkonen, M;Torsti, T;Skottman, H;Kauppinen, A;Nymark, S;Kaarniranta, K;
The Journal of biological chemistry
Species: Human
Sample Types: Whole Cells
Applications: ICC
Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE
Authors: T Viheriälä, J Sorvari, TO Ihalainen, A Mörö, P Grönroos, S Schlie-Wol, B Chichkov, H Skottman, S Nymark, T Ilmarinen
Scientific Reports, 2021-01-13;11(1):933.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Human RPE stem cells grown into polarized RPE monolayers on a polyester matrix are maintained after grafting into rabbit subretinal space.
Authors: Stanzel B, Liu Z, Somboonthanakij S, Wongsawad W, Brinken R, Eter N, Corneo B, Holz F, Temple S, Stern J, Blenkinsop T
Stem Cell Reports, 2014-01-02;2(1):64-77.
Species: Human
Sample Types: Whole Cells
Applications: ICC