Human Claudin-3 Fluorescein-conjugated Antibody

Catalog #: FAB4620F Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4620F

Detection of Claudin‑3 in PC‑3 Human Cell Line by Flow Cytometry.

1 Image

All Claudin-3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-3 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human Claudin-3 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 385021

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Claudin-3
Met1-Val220
Accession # O15551

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Claudin‑3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4620F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-3

Claudin-3 is a 23 kDa multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-3 is upregulated by EGF and in inflammation and a variety of epithelial cancers. Its expression is lost in the blood brain barrier during pathological disruptions of that structure. Claudin-3 binding to the Clostridium perfringes exotoxin induces epithelial cell lysis. Human Claudin-3 shares 91% amino acid sequence identity with mouse and rat Claudin-3.

Entrez Gene IDs

1365 (Human); 12739 (Mouse); 65130 (Rat)

Alternate Names

C7orf1; claudin 3; Claudin3; Claudin-3; CLDN3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-R2; CPETR2; CPETR2CPE-receptor 2; HRVP1; Rat ventral prostate.1 protein homolog; RVP1; ventral prostate.1 protein homolog; ventral prostate.1-like protein