Human CLEC-2/CLEC1B Antibody Summary
CLEC-1 is observed.
Gln58-Pro229
Accession # AAF36777
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CLEC-2/CLEC1B by Western Blot. Western blot shows lysates of human platelets. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CLEC-2/CLEC1B Monoclonal Antibody (Catalog # MAB1718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC-2/CLEC1B at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC-2/CLEC1B
C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet-aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).
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Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
- Christou, C.M. et al. (2008) Biochem. J. 411:133.
- Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
- Suzuki-Inoue, K. et al. (2006) Blood 107:542.
- Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
- Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
- Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
- Chaipan, C. et al. (2006) J. Virol. 80:8951.
Product Datasheets
Citations for Human CLEC-2/CLEC1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
Cell Death Dis, 2017-08-24;8(8):e3017.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Inhibitory effects of polypeptides derived from a snake venom C-type lectin, aggretin, on tumor cell-induced platelet aggregation.
Authors: Chang C, Chung C, Hsu C, Peng H, Huang T
J Thromb Haemost, 2014-04-01;12(4):540-9.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
A novel mechanism of cytokine release in phagocytes induced by aggretin, a snake venom C-type lectin protein, through CLEC-2 ligation.
Authors: Chang CH, Chung CH, Hsu CC, Huang TY, Huang TF
J. Thromb. Haemost., 2010-11-01;8(11):2563-70.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
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