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Human CLP24 Antibody

Catalog #: MAB6747 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6747
MAB6747-SP
Detection of CLP24 in CLP24 and eGFP Co-Transfected Cells by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLP24 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLP24 in ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 652424
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CLP24
Accession # Q9BSN7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CLP24 antibody in CLP24 and eGFP Co-Transfected Cells antibody by Flow Cytometry. View Larger

Detection of CLP24 in CLP24 and eGFP Co-Transfected Cells by Flow Cytometry. Human CLP24 and eGFP co-transfectants were stained with Mouse Anti-Human CLP24 Monoclonal Antibody (Catalog # MAB6747) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Immunocytochemistry CLP24 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

CLP24 in NTera‑2 Human Cell Line. CLP24 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Mouse Anti-Human CLP24 Monoclonal Antibody (Catalog # MAB6747) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLP24

TMEM204, also known as Claudin-like protein of 24 kDa (CLP24), is a cell surface protein with four transmembrane domains and a C-terminal PDZ motif. TMEM204 localizes to adherens junctions between epithelial and endothelial cells. It is involved in regulating paracellular flux and is upregulated under hypoxic conditions. Human TMEM204 shares 88% aa sequence identity with mouse and rat TMEM204.

Long Name
Claudin-like Protein 24
Entrez Gene IDs
79652 (Human); 407831 (Mouse); 287129 (Rat)
Alternate Names
C16orf30; C16orf30chromosome 16 open reading frame 30; Claudin-like protein 24; CLP24; CLP24MGC111564; FLJ20898; TMEM204; transmembrane protein 204

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