Human Coagulation Factor XI Light Chain Antibody

Catalog #: MAB24601 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24601
MAB24601-SP

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Human Coagulation Factor XI Light Chain Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor XI Light Chain in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) UPA-1, rhThrombin, rhKLK1, rhKLK3, rhKLK4, rhKLK5, rhKLK6, rhKLK8, rhKLK11, rhHGFA, rhFactor VII, or rhFactor X is observed. This antibody recognizes mature chain (aa 19‑625), light chain (aa 388‑625) and the active enzyme, which contains disulfide bond-linked light and heavy chains.

Source

Monoclonal Mouse IgG_2B Clone # 318104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor XI
Glu19-Val625
Accession # P03951

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Coagulation Factor XI (Catalog # 2460-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor XI (Catalog # 2460-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Coagulation Factor XI (2 µg/mL, Catalog # 2460-SE) cleavage of the fluorogenic peptide substrate Boc-IEGR-Amc(100 µM). The Neutralization Dose (ND₅₀) is typically 2.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor XI

Coagulation factors XI and XIa refer to the pro and active forms of the same protease, respectively (1). Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa. Factor Xa in turn activates factor II/thrombin to complete the coagulation cascade. Patients with factor XI deficiency are prone to excessive bleeding after hemostatic challenge. There are two alternative splicing forms. Isoform 1 corresponds to the circulating plasma factor XI and isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells (2). The 625 amino acid (aa) precursor of isoform 1 consists of a signal peptide (aa 1-18) and the mature chain (aa 19-625). The mature chain (XI) can be further processed into the heavy chain (aa 19-387) and the light chain (aa 388-625) (XIa).

References