Human Complement Component C1r Biotinylated Antibody

Catalog #: BAF1807 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF1807

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Component C1r Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Component C1r in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human Complement Component C1s is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
Ser18-Asp705
Accession # NP_001724

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Complement Component C1r (Catalog # 1807-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1r

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑705) of human C1r was expressed, which had the Leu152 natural variant (3). The purified protein corresponded to the processed active form, with A and B chains starting at residue Ser18 and Ile464, respectively.

References

Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
Journet A. and M. Tosi (1986) Biochem. J. 240:783.

Entrez Gene IDs

715 (Human)

Alternate Names

C1r; complement C1r subcomponent; Complement component 1 subcomponent r; complement component 1, r subcomponent; Complement Component C1r; EC 3.4.21; EC 3.4.21.41