Human Complement Component C5/C5a Antibody Summary
Thr678-Arg751
Accession # P01031
Applications
under non-reducing conditions only
Human Complement Component C5/C5a Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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N-Acetyl-beta -D-Glucosamini-dase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a Antibody. Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosamin-idase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human Complement Component C5a Monoclonal Antibody (Catalog # MAB2037). The ND50 is typically 0.04-0.12 µg/mL in the presence of cytochalasin-B.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5/C5a
Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
- Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
- DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
- Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
- Okinaga, S. et al. (2003) Biochemistry 42:9406.
- Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Product Datasheets
Citations for Human Complement Component C5/C5a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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The Influence of an Elastase-Sensitive Complement C5 Variant on Lupus Nephritis and Its Flare
Authors: CR Toy, H Song, HN Nagaraja, J Scott, J Greco, X Zhang, CY Yu, JA Tumlin, BH Rovin, LA Hebert, DJ Birmingham
Kidney international reports, 2021-06-09;6(8):2105-2113.
Species: Human
Sample Types: Plasma
Applications: ELISA Capture -
Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy
Authors: TA Shah, HK Pallera, CL Kaszowski, WT Bass, FA Lattanzio
Frontiers in Neuroscience, 2021-02-12;15(0):616734.
Species: Rat
Sample Types: Plasma
Applications: ELISA Capture -
Establishing a Case for Anti-complement Therapy in Membranous Nephropathy
Authors: I Ayoub, JP Shapiro, H Song, XL Zhang, S Parikh, S Almaani, S Madhavan, SV Brodsky, A Satoskar, C Bott, L Yu, M Merchant, J Klein, JM Mejia-Vile, T Nadasdy, D Birmingham, BH Rovin
Kidney international reports, 2020-12-13;6(2):484-492.
Species: Human
Sample Types: Urine
Applications: ELISA Capture -
Attenuation of Staphylococcus aureus-Induced Bacteremia by Human Mini-Antibodies Targeting the Complement Inhibitory Protein Efb.
Authors: Georgoutsou-Spyridonos M, Ricklin D, Pratsinis H, Perivolioti E, Pirmettis I, Garcia B, Geisbrecht B, Foukas P, Lambris J, Mastellos D, Sfyroera G
J Immunol, 2015-09-04;195(8):3946-58.
Species: Human
Sample Types: Plasma
Applications: ELISA Development (Capture) -
Generation of multiple fluid-phase C3b:plasma protein complexes during complement activation: possible implications in C3 glomerulopathies.
Authors: Ramadass, Mahalaks, Ghebrehiwet, Berhane, Smith, Richard, Kew, Richard
J Immunol, 2013-12-23;192(3):1220-30.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture) -
C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome.
Authors: Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD
J. Immunol., 2008-06-01;180(11):7368-75.
Species: Human
Sample Types: BALF
Applications: ELISA Development
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