Home / Complement Factor H-related 4/CFHR4 / Human Complement Factor H-related 4/
CFHR4 Antibody

Human Complement Factor H-related 4/
CFHR4 Antibody

Catalog #: MAB5980
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5980
MAB5980-SP
Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor H-related 4/
CFHR4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CFHR1, rhCFHR2, or rhCFHR5 is observed.
Source
Monoclonal Mouse IgG1 Clone # 640212
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4
Glu20-Glu331
Accession # Q92496
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Complement Factor H-related 4/CFHR4 antibody by Western Blot. View Larger

Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H-related 4/ CFHR4 at approximately 45 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Complement Factor H-related 4/CFHR4 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

Complement Factor H-related 4/CFHR4 in Human PBMCs. Complement Factor H-related 4/CFHR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMC) using Human Complement Factor H-related 4 Monoclonal Antibody (Catalog # MAB5980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Complement Factor H-related 4/CFHR4 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Complement Factor H-related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 4/CFHR4

FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.

Entrez Gene IDs
10877 (Human)
Alternate Names
complement factor H-related protein 4; CFHL4; CFHR4; Complement Factor Hrelated 4; Complement Factor H-related 4; FHR4; FHR-4

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Citation for Human Complement Factor H-related 4/
CFHR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Factor H-related protein 4 activates complement by serving as a platform for the assembly of alternative pathway C3 convertase via its interaction with C3b protein.
    Authors: Hebecker M, Jozsi M
    J. Biol. Chem., 2012-04-19;287(23):19528-36.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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CFHR4 Antibody

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