Human Contactin-3 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Contactin-3 antibody in Human Brain by Immunohistochemistry (IHC-P). Contactin-3 antibody in Human Brain by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/Contactin-3_AF7125_Immunohistochemistry_11519.jpg)
Contactin‑3 in Human Brain. Contactin-3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7125) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Background: Contactin-3
Contactin-3, also known as BIG-1, CNTN3 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).
- Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
- Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
- Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
- Yoshihara, Y. et al. (1994) Neuron 13:415.
- Kamei, Y. et al. (2000) Genomics 69:113.
- Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
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