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Human CXCL16 ELISA Kit - Quantikine

Catalog #: DCX160
14 Citations Datasheet / COA / SDS
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DCX160
Human CXCL16 ELISA Standard Curve
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Product Details
Procedure
Citations (14)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CXCL16 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.017 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human CXCL16
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CXCL16 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CXCL16 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CXCL16 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CXCL16 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CXCL16.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 42 42
Mean (pg/mL) 0.92 2.85 6.33 1 3.3 7.3
Standard Deviation 0.04 0.1 0.31 0.1 0.3 0.7
CV% 4.3 3.5 4.9 10 9.1 9.6

Recovery

The recovery of CXCL16 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-110
EDTA Plasma (n=4) 102 94-112
Heparin Plasma (n=4) 97 85-105
Serum (n=4) 103 89-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CXCL16 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL16 ELISA Linearity

Scientific Data

N/A Human CXCL16 ELISA Standard Curve View Larger

Human CXCL16 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.

Entrez Gene IDs:
58191 (Human); 66102 (Mouse)
Alternate Names:
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CXCL16 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Platelet SR-PSOX/CXCL16-CXCR6 Axis Influences Thrombotic Propensity and Prognosis in Coronary Artery Disease
    Authors: T Guan, F Emscherman, C Schories, P Groga-Bada, P Martus, O Borst, M Gawaz, T Geisler, D Rath, M Chatterjee
    International Journal of Molecular Sciences, 2022-09-21;23(19):.
    Species: Human
    Sample Types: Plasma
  2. Two New Potential Therapeutic Approaches in Radiation Cystitis Derived from Mesenchymal Stem Cells: Extracellular Vesicles and Conditioned Medium
    Authors: C Helissey, N Guitard, H Théry, S Goulinet, P Mauduit, M Girleanu, AL Favier, M Drouet, C Parnot, C Chargari, S Cavallero, S François
    Biology, 2022-06-28;11(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. High miR-451 expression in peripheral blood mononuclear cells from subjects at risk of developing rheumatoid arthritis
    Authors: K Prajzlerov, O Kryšt?fkov, P Hánová, V Horváthová, M Gregová, K Pavelka, J Vencovský, L Šenolt, M Filková
    Scientific Reports, 2021-02-25;11(1):4719.
    Species: Human
    Sample Types: Plasma
  4. Role of CXCL16 in BLM-induced epithelial-mesenchymal transition in human A549 cells
    Authors: Z Ma, C Ma, Q Zhang, Y Bai, K Mu, X Liu, Q Yang
    Respiratory Research, 2021-02-06;22(1):42.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
    Kidney Int Rep, 2019-06-21;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  6. Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
    Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
    Oncotarget, 2017-07-07;8(37):62081-62098.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Wnt5a-Ror2 signaling in mesenchymal stem cells promotes proliferation of gastric cancer cells by activating CXCL16-CXCR6 axis.
    Authors: Gosuke Takiguchi, Michiru Nishita, Kana Kurita, Yoshihiro Kakeji, Yasuhiro Minami
    Cancer Science, 2016-02-09;0(0):1349-7006.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Suppression of NF-kappaB Reduces Myocardial No-Reflow.
    Authors: Zeng M, Yan H, Chen Y, Zhao H, Lv Y, Liu C, Zhou P, Zhao B
    PLoS ONE, 2012-10-09;7(10):e47306.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221.
    Authors: Santhekadur PK, Das SK, Gredler R, Chen D, Srivastava J, Robertson C, Baldwin AS, Fisher PB, Sarkar D
    J. Biol. Chem., 2012-03-06;287(17):13952-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. An intron polymorphism in the CXCL16 gene is associated with increased risk of coronary artery disease in Chinese Han population: a large angiography-based study.
    Authors: Huang M, Han Y, Zhang X
    Atherosclerosis, 2009-11-10;210(1):160-5.
    Species: Human
    Sample Types: Plasma
  11. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
    Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
    Cancer Res., 2008-12-01;68(23):9996-10003.
    Species: Human
    Sample Types: Serum
  12. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
    Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
    Int. J. Oncol., 2008-08-01;33(2):297-308.
    Species: Human
    Sample Types: Serum
  13. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
    Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
    Glia, 2008-05-01;56(7):764-74.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins.
    Authors: Smith C, Halvorsen B, Otterdal K, Waehre T, Yndestad A, Fevang B, Sandberg WJ, Breland UM, Froland SS, Oie E, Gullestad L, Damas JK, Aukrust P
    Cardiovasc. Res., 2008-03-13;79(1):195-203.
    Species: Human
    Sample Types: Cell Culture Supernates

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